Assembly

  1. Mount sample chamber

  2. Attach cage rods for illumination and detection arms to sample chamber

  3. Mount objective lenses using silicone

  4. Start assembling the detection arm

  5. Adjust distance camera - tube lens by focusing to infinity

  6. Finalize detection arm

  7. Assemble motor unit and sample holder

  8. Assemble illumination arm with mirrors, but without lenses

  9. Align laser to center of cage system and sample chamber

  10. Add circular lenses step by step, adjust focus and collimation where necessary

  11. Add cylindrical lens and adjust light sheet to focal plane of detection objective

  12. Add laser shielding to breadboard