Suggested modifications


  1. Improve light sheet alignment stability by switching from Thorlabs KC45D to Thorlabs C45P
    Not gimbal; requires normal/thicker mirrors (compared to KC45D); might run into space issues

  2. Integrate a bio-compatible sample holder, made from e.g. plastic or surgical-grade stainless steel
    Requires new sample holder design, different adapter

  3. Switch camera to an sCMOS USB camera with similar pixel size and larger chip to reduce the required computer size / enable the use of a laptop
    Requires substantial adaptation of the software, or use of different software, e.g. µManager

  4. Switch to µManager to make system more universal
    Requires integration of all hardware components in µManager, which might be difficult for some components, e.g. Thorlabs APT stage

  5. Use lasers that support direct modulation to skip AOTF, simplify illumination beam path and reduce footprint of electronics rack
    Requires modification of software, modification of illumination beam path